Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering
نویسندگان
چکیده
In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that obtained in standard quartz cuvettes. Then, we have carried out DLS measurements of a counter-diffusion crystallization experiment of glucose isomerase in capillaries of different diameters (0.1, 0.2 and 0.3 mm) in order to follow the temporal evolution of protein supersaturation. Finally, we have compared DLS data with optical recordings of the progression of the crystallization front and with a simulation model of counter-diffusion in 1D.
منابع مشابه
Protein Crystallization Screening through Scattering Techniques
We have employed dynamic light scattering and X-ray small-angle scattering for monitoring the cluster formation of hen-egg white lysozyme solutions under crystallization conditions. From time-resolved dynamic light scattering experiments we can obtain a set of observables that exhibit clear extrema, when simultaneously plotted as functions of protein and electrolyte molarity. Close to these ext...
متن کاملEffect of tocopherol on Pluronic microemulsions: turbidity studies and Dynamic light scattering and dynamic surface tension measurements
The development and design of the biocompatible and biodegradable thermodynamically stable micellar and microemulsion transparent dispersions to reduce the free and unbounded drugs concentration in the blood is a basic challenge in field of drug efficacy and bioavailability of drugs. In the current work, solubilization capacity of the drug (Tocopherol), oil (Ethyl Butyrate), and oil+drug (1:1 m...
متن کاملCrystallization of biological macromolecules using agarose gel.
Gellified media prevent convection and crystal sedimentation, and provide an attractive growth environment for optimising biological crystals. Agarose gels are particularly easy to use and they are compatible with most of the common crystallization methods. They also offer new possibilities like counter-diffusion techniques. This paper gives a brief overview of their general properties and pres...
متن کاملIn situ data collection and structure refinement from microcapillary protein crystallization.
In situ X-ray data collection has the potential to eliminate the challenging task of mounting and cryocooling often fragile protein crystals, reducing a major bottleneck in the structure determination process. An apparatus used to grow protein crystals in capillaries and to compare the background X-ray scattering of the components, including thin-walled glass capillaries against Teflon, and var...
متن کاملEntrapment–D-(+)-Glucose Water Nanodroplet: Synthesis and Dynamic Light Scattering
In the present research, the D-(+)-Glucose–confined water nanodroplets with mean size in the range of 6-11 nm were synthesized at D-(+)-Glucose different concentrations by AOT reverse micelles (RMs) method as a function of mass fraction of droplet (MFD) at the constant water-to-surfactant molar ratio ( W=40). The dynamic light scattering of the nano-sized water droplets containing the D-(+)-Glu...
متن کامل